Figure 2 | Scientific Reports

Figure 2

From: Subtilisin-like protease-1 secreted through type IV secretion system contributes to high virulence of Streptococcus suis 2

Figure 2

Direct interaction between SspA-1 and VirD4–89K.

(A) Pull-down assay between GST–VirD4 and purified His–SspA-1. GST–VirD4 or GST alone was pre-absorbed with glutathione beads and mixed with purified His-SspA-1 or BSA. GST or BSA was used as a negative control for bait or prey, respectively. (B) Pull-down assay between GST–VirD4 and native SspA-1 in the ΔvirD4–89K mutant. GST–VirD4 or GST alone was pre-absorbed with glutathione beads and then mixed with the total proteins from ΔvirD4–89K or ΔsspA-1 lysates. GST or ΔsspA-1 lysates were used as negative controls for the bait or prey, respectively. Pull-down samples and input samples (0.1% input His-SspA-1 or 2% input ΔvirD4–89K lysates) were subjected to Western blot with anti-SspA-1 or anti-GST.

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