Figure 1: NTP inhibits the expression of CCL17 in keratinocytes.

(a) A schematic diagram describing the NTP producing device (left panel) and the pictures of NTP device (right panel). (b) Treating time (plasma dose)-dependent effect of NTP on CCL17 expression. GO: gas only (argon gas), nt: non-treated cells. (c) Time-dependent CCL17 mRNA expression pattern after NTP treatment for 5 min. *p < 0.05 (d) A drawing describing the 3 different methods used for the treatment of HaCaT cells with NTP. (e) The differences among 3 NTP treatment methods in their effect on CCL17 expression by the cells. (f) The lifetime of NTP’s activity in influencing cellular CCL17 expression. Equal amounts of media (2 ml each) were treated with NTP for 5 min, then incubated in a cell culture chamber for 0, 2, and 4 h before being used for the treatment of cells. The data shown are representative of 3 separate experiments.