Figure 5: NTP treatment inhibits the DNCB-induced activation of NF-κB in the mouse skin tissues.

(a) The results of RT-PCR and western blot analyses of mouse skin tissues. The data shown are representative of 4 independent experiments. (b) The results of an immunofluorescence (IF) assay against p65/RelA. The number of nuclei showing co-localization with p65/RelA staining were calculated by counting the nuclei stained in purple in the epidermis and averaged for each mouse using 5 randomly selected pictures. Scale Bar: 50 μm. (c) The results of ELISA analysis for the detection of CCL17, IgE, and IFNγ proteins in skin tissues. The data shown are representative of 4 independent experiments, *p < 0.05.