Table 1 Sequence and biophysical properties of AR-23 and its analogues.

Peptide	Sequence	Calculated mass MW^a	Observed mass MW^a	Net charge	uH^b	tR (min)^c	Stabilization energy (kcal/mol)^d
Melittin	GIGAVLKVLTTGLPALISWIKRKRQQ.NH₂	2844.75	2845.4	6	0.499	19.14	−31.72
RV-23	RIGVLLARLPKLFSLFKLMGKKV.NH₂	2625.68	2626.4	7	0.618	11.05	−29.63
AR-23	AIGSILGALAKGLPTLISWIKNR.NH₂	2390.45	2391.3	4	0.548	16.6	−32.84
A(A1R)	RIGSILGALAKGLPTLISWIKNR.NH₂	2475.52	2476.2	5	0.548	15.69	−24.31
A(A8R)	AIGSILGRLAKGLPTLISWIKNR.NH₂	2475.52	2476.5	5	0.709	19.61	−26.52
A(I17K)	AIGSILGALAKGLPTLKSWIKNR.NH₂	2405.46	2406.0	5	0.512	8.0	−29.83
A(I17R)	AIGSILGALAKGLPTLRSWIKNR.NH₂	2433.45	2434.2	5	0.566	10.67	−23.18
A(A1R, A8R)	RIGSILGRLAKGLPTLISWIKNR.NH₂	2560.58	2561.4	6	0.754	6.53	−25.73
A(A1R, I17K)	RIGSILGALAKGLPTLKSWIKNR.NH₂	2490.53	2491.2	6	0.512	5.16	−27.54
A(A8R, I17K)	AIGSILGRLAKGLPTLKSWIKNR.NH₂	2490.53	2491.5	6	0.704	6.59	−27.93
A(A1R, A8R, I17K)	RIGSILGRLAKGLPTLKSWIKNR.NH₂	2575.59	2576.1	7	0.754	12.23	−31.72
A(A1R, A8R, I17R)	RIGSILGRLAKGLPTLRSWIKNR.NH₂	2603.58	2604.4	7	0.754	10.78	−29.63

^aMolecular weight (MW) as measured by mass spectroscopy (MS).
^bμH, mean relative hydrophobic moment determined at website: http://www.bioinformatics.nl/emboss-explorer/.
^ctR, retention time measured by reverse-phase HPLC.
^dThe stabilization energy of peptides was calculated using InsightII2000 software based on the computer-guided homology method.

Quick links

Search