Figure 2: Altered protein level of GABAergic neurons in the adolescent and adult in vivo VPA model. | Scientific Reports

Figure 2: Altered protein level of GABAergic neurons in the adolescent and adult in vivo VPA model.

From: Distinct Defects in Synaptic Differentiation of Neocortical Neurons in Response to Prenatal Valproate Exposure

Figure 2

(a,b) Differential effect of prenatal VPA-exposure on expression of inhibitory neuronal cell-type specific proteins, Parvalbumin (PV) and Calretinin (CR), in adolescent (n = 33–38 animals from 9–11 VPA dams; n = 33–41 animals from 7–9 control dams) and adult mice (n = 29–41 animals from 9–13 VPA dams; n = 30–38 animals from 12–13 control dams). Blots were cropped to display the relevant molecular weight range. Uncropped blots are presented in Fig. S4c. (c,d) Number of PV-positive inhibitory interneurons in adolescent mice exposed to VPA in utero. Immunohistochemistry on coronal sections from somatosensory cortex were performed with anti-PV and anti-GAD67 antibodies (some double-positive cell somata are highlighted with arrow heads). 32 and 54 confocal images were analyzed from 3 control and 4 VPA animals, respectively. Scale bar = 50 μm.

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