Figure 3: In vitro VPA exposure into cultured cortical neurons during early development recapitulates altered expression of presynaptic proteins in adolescent in vivo VPA model. | Scientific Reports

Figure 3: In vitro VPA exposure into cultured cortical neurons during early development recapitulates altered expression of presynaptic proteins in adolescent in vivo VPA model.

From: Distinct Defects in Synaptic Differentiation of Neocortical Neurons in Response to Prenatal Valproate Exposure

Figure 3

(a) Scheme for the procedure of in vitro VPA exposure. (b,c) Example western blots and quantification of relative expression levels of synaptic proteins in cultured neocortical neurons treated with VPA for first 6 days of culture (n = 5–23). The % change in expression levels relative to data from vehicle treated control cultures is displayed. Blots were cropped to display the relevant molecular weight range. Uncropped blots are presented in Fig. S4d. (d) Effects of duration of VPA treatment. Neurons were treated with 2 mM VPA for 0, 3 and 6 days. A value in the untreated (0 h) neurons was arbitrarily defined as 0. (n = 3–4). (e) Effects of VPA concentrations in cultured medium. Neurons were treated with different concentration of VPA (0, 1, 1.5 and 2 mM) for 6 hrs (n = 3–6). (f) Timing-dependent effect of VPA treatment. Neurons were treated with 2 mM VPA for 6 days from DIV0 or DIV9 (n = 4).

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