Figure 5: Increased spontaneous synaptic activity and alterations in mEPSC and mIPSC frequency in VPA treated neurons in-vitro. | Scientific Reports

Figure 5: Increased spontaneous synaptic activity and alterations in mEPSC and mIPSC frequency in VPA treated neurons in-vitro.

From: Distinct Defects in Synaptic Differentiation of Neocortical Neurons in Response to Prenatal Valproate Exposure

Figure 5

(a,b) VPA treated neurons show a high frequency of spontaneous synaptic currents with large amplitude indicating increased synaptic network activity compared to untreated neurons (n = 5 cells per condition). (c) mEPSCs (left) and mIPSCs (right) were recorded in untreated (upper traces) and VPA treated (lower traces) neurons in the presence of 0.5 μM TTX and 2 μM gabazine or 5 μM CNQX plus 25 μM AP5, respectively. (d) In VPA treated neurons (n = 15), the frequency of mEPSCs is significantly increased relative to control (n = 21) whereas the frequency of mIPSCs is significantly decreased (n = 19) as compared to untreated neurons (n = 22). The amplitude of mEPSCs and mIPSCs is not significantly changed between untreated and treated neurons. (e) Cumulative probability distribution of interevent interval of mEPSCs (upper panel) and mIPSCs (lower panel) obtained from the same cells as in (d).

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