Figure 6: In vitro VPA treatment alters expression of GAD65 and vGluT1 through selective HDAC inhibition. | Scientific Reports

Figure 6: In vitro VPA treatment alters expression of GAD65 and vGluT1 through selective HDAC inhibition.

From: Distinct Defects in Synaptic Differentiation of Neocortical Neurons in Response to Prenatal Valproate Exposure

Figure 6

(a) Scheme of procedure of experiments using a HDAC inhibitor, MS275. (b,c) Example western blots and quantification of relative changes in protein level in cultured neocortical neurons treated with MS275 (n = 4–9) or VPA (n = 6–11) as compared to vehicle treated control cultures. Combined blots were run under the same experimental conditions and blots were cropped to display the relevant molecular weight range. Uncropped blots are presented in Fig. S4e. (d) Altered mRNA level in cultured cortical neurons treated with MS275 (n = 13) or VPA (n = 11).

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