Figure 6 | Scientific Reports

Figure 6

From: Specific threonine-4 phosphorylation and function of RNA polymerase II CTD during M phase progression

Figure 6

CTD Thr4 mutants inhibit proper M phase progression.

The phenotype of CTD mutants was studied in human B cell line Raji after expression of CTD mutants. Removal of tetracycline induced expression of (a) recombinant Rpb1 CTD mutants. (b) Immunofluorescence images of γ-tubulin (green) and DNA (DAPI) signals 24 h after induction. (c) Percentage of normal and abnormal cells, with polyploid or lobed nuclei was determined. Data are from three experiments; n = 450, number of cells analyzed for each mutant. Error bars represent standard deviations. (d) The HA mAb allows the visualization of the distribution of recombinant Pol II in cells (red). Arrows indicate a strong enrichment of Pol II in two distinct foci symmetrically to condensed chromatin in metaphase cells. Scale bars, 5 μm.

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