Figure 5
From: Programmable DNA Nanosystem for Molecular Interrogation
Programming OPTIMuS for nucleic acid detection.
(a) Cross-section of the interface displaying the placement of the target-associated duplexes. Five sites were remodeled to contain unique duplexes such that the toehold-bearing strand emerged from a staple on the ring and its complement emerged from frameR, as shown in the 3D rendering. The duplex contributes to ring-frameR adhesion. The remaining active sites were modified to the no blunt end state, as described in Fig. 3. (b) The basic scheme of toehold-mediated DNA strand displacement. A target strand invades the duplex to hybridize with the toehold-bearing strand, which allows the two components to separate from each other. (c) FRET readout in different configurations showing that under force induction the ring/frameR interface is disrupted only in the presence of soluble target oligonucleotide (*Indicates P ≤ 0.05).
