Figure 6 | Scientific Reports

Figure 6

From: Thyrostimulin-TSHR signaling promotes the proliferation of NIH:OVCAR-3 ovarian cancer cells via trans-regulation of the EGFR pathway

Figure 6

The effects of thyrostimulin-TSHR signaling on RTK expression and EGFR activation in NIH:OVCAR-3 cells.

(A) The cells treated without or with thyrostimulin for 1 day were harvested for cDNA extraction and real-time quantification of the indicated RTKs. *P < 0.05; ***P < 0.001. To evaluate the trans-regulation of EGFR, the cells treated with or without thyrostimulin for the indicated time intervals were harvested for (B) the real-time quantification of the EGFR transcript, (C) the detection of the total amount of EGFR protein present or (D) the detection of the phosphorylated EGFR levels. For real-time PCR, data were normalized against β-actin and are shown as the mean ± SD. For Western blot quantification, the blots were assessed by densitometer using β-actin as a loading control. The results are shown as the mean ± SE of three independent experiments. One representative image of the blot was shown below.

Back to article page