Figure 2: Effect of soluble cholesterol on GLP-1R mediated cAMP generation. | Scientific Reports

Figure 2: Effect of soluble cholesterol on GLP-1R mediated cAMP generation.

From: Restoring Mitochondrial Function: A Small Molecule-mediated Approach to Enhance Glucose Stimulated Insulin Secretion in Cholesterol Accumulated Pancreatic beta cells

Figure 2

(A) GLP-1R mediated cAMP generation in pancreatic beta cells treated with different concentration of cholesterol for 12 h and cAMP generated was measured by luciferase assay(CRE: cyclic AMP responsive element). (B) PKA substrate phosphorylation profile in normal and cholesterol treated pancreatic beta cells; C: cholesterol treated, C + E: cholesterol and Exendin-4 treated, N: Untreated; N + E: Exendin-4 treated. (C) Time course analysis of the effect of soluble cholesterol on basal and GLP-1R mediated cAMP generation. The cells were treated with 160 μM cholesterol for 1 h (C(i)), 6 h (C(ii)), 12 h (C(iii)) and 24 h (C(iv)) following which treated with GLP-1R agonist and cAMP generated was measured by luciferase assay. (D) Time course analysis of the effect of soluble cholesterol on survival of BRIN-BD11 pancreatic beta cells: the cells were treated with 160 μM soluble cholesterol for 6 h (D(i)), 12 h (D(ii)) and 24 h (D(iii)), following which the cell survival was measured by MTT assay. Data are shown as mean ± SEM, n ≥ 3; *P < 0.05, **P < 0.01, ***P < 0.001 t-test (unpaired), ns: not significant; Ex-4: Exendin-4; chol: cholesterol; M1: small molecule M1.

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