Figure 3 | Scientific Reports

Figure 3

From: Modulation of oxidative stress and subsequent induction of apoptosis and endoplasmic reticulum stress allows citral to decrease cancer cell proliferation

Figure 3

Citral induces apoptosis.

A, To monitor cell death via apoptosis, ECC-1 (upper panel) and OVCAR-3 (lower panel) cells were treated overnight with designated concentrations of citral. After treatment, cells were labeled with Annexin-V-FITC and propidium iodide. Apoptotic cell death in the cells was monitored by flow cytometry. Data presented is representative of three independent experiments. B, Citral induced apoptosis in ECC-1 and OVCAR-3 cells was confirmed by monitoring for cleaved caspase-3 and expression of Bax and Bcl-2 by western blotting. Lysates of ECC-1 and OVCAR-3 cells treated with citral (25 and 50 μM, respectively) for 0, 24 and 48 h were used in the Western blot analysis. Data shown is representative of three independent experiments. C, Annexin-V-FITC and propidium iodide staining assays do not show an increase in apoptotic SKOV-3 cells treated for 48 h with 50 μM citral. Representative dot plots are shown. Bar graph is data from three independent experiments.

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