Table 3 Descriptive parameters (mean ± SEM) for the two clusters obtained following two-step cluster analysis using all sperm samples collectively.

From: Does advancing male age influence the expression levels and localisation patterns of phospholipase C zeta (PLCζ) in human sperm?

PLCζ

Cluster 1

Cluster 2

N

47

24

% Sperm exhibiting PLCζ

88.9 ± 1.2

36.9 ± 5.5

Total levels of PLCζ

37.2 ± 2.3

22.6 ± 2.6

Localisation patterns (%)

 A

1.4 ± 0.4

2.4 ± 1.0

 PA

7.4 ± 1.4

7.9 ± 1.9

 E

29.3 ± 3.8

10.8 ± 2.9

 A + PA

1.4 ± 0.5

1.3 ± 0.6

 A+ E

3.5 ± 1.1

1.0 ± 0.4

 PA+ E

29.9 ± 3.9

8.5 ± 1.8

 A + PA +E

15.7 ± 2.6

10.6 ± 3.0

 None

11.3 ± 1.1

52.7 ± 5.8

  1. [Specific localisation patterns of PLCζ are referred to as: A = acrosomal; E = equatorial; None = absence of PLCζ; PA = post-acrosomal. ‘None’ refers to sperm that were completely devoid of PLCζ].
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