Figure 3: Vessel formation by endothelial EA.hy926 cells and co-culture of EA. hy926 and U-87 MG cells on a Matrigel layer.
From: Antithrombin controls tumor migration, invasion and angiogenesis by inhibition of enteropeptidase
Vessel formation was evaluated in different conditions after 24 hours of incubation. (A) Vessel formation by endothelial cells. (B) Vessel formation by co-culture of endothelial and U-87 MG cells. D,L-sulforaphane was used as positive control. Images were recorded with a Leica microscope at 5×, and ImageJ was used to analyze angiogenesis. Statistical analysis was carried out with a Mann-Whitney U test. Each condition was evaluated in octuplicate. *p < 0.05; **p < 0.01. AT: antithrombin, LMWH: low molecular weight heparin; AT-EP-LMWH: mix of antithrombin, enteropeptidase and low molecular weight heparin after 24 hours of incubation, when enteropeptidase is completely inhibited and antithrombin is completely cleaved.
