Figure 5: Effects of antithrombin and heparin on invasion by a transwell assay.
From: Antithrombin controls tumor migration, invasion and angiogenesis by inhibition of enteropeptidase
Cell invasion was evaluated after incubation of U-87 MG cells for 6 hours under the following conditions: no treatment, incubation with low molecular weight heparin (LMWH), antithrombin (AT) or incubation with antithrombin and LMWH in combination (AT-LMWH). (A) Microscope images of cells invaded after 6 hours of incubation. (B) Percentage of cells invading under the different conditions. Each condition was evaluated in triplicate, and three different images were processed for each different assay; *p < 0.05; **p < 0.01. Images were recorded with a Leica microscope at 5×, and Image J was used to analyze cells that had migrated. Statistical analysis was carried out with a Mann-Whitney U test.
