Figure 2

Renin overexpression promotes T_H17 response and gut epithelial cell apoptosis in colitis model.

(A–D) Lamina propria cells isolated from WT and RenTg mice on day 2 after TNBS treatment were analyzed by flow cytometry. Control mice received ethanol only. (A) Representative FACS plots gated on CD4⁺TCRβ⁺ cells. (B) Quantitation of INF-γ⁺ T cells; (C) Quantitation of IL-17⁺ T cells; (D) Quantitation of INF-γ⁺IL-17⁺ T cells. ***P < 0.001; n = 3–4 in each group. (E) Representative TUNEL staining of WT and RenTg distal colon sections on day 4 after TNBS treatment. Original magnification 100x. (F) Western blot analyses and densitometric quantitation of colonic mucosal PUMA, p53 and activated caspase 3 proteins in untreated and TNBS-treated WT and RenTg mice on day 2. **P < 0.01, ***P < 0.001 vs. Ctrl; ^##P < 0.05 vs. corresponding TNBS-treated colons. All gels were run under the same experimental condition and gel images are cropped for concise presentation. All uncut gel images are provided in Supplementary Information.