Figure 3

Renin inhibition ameliorates the severe colitis in RenTg mice.

RenTg mice were pre-treated with PBS or aliskiren (10 mg/kg, i.p. daily) for one week before TNBS instillation. Aliskiren or PBS treatment continued after TNBS treatment. (A) Body weight changes (% of original body weight) over time (days) following TNBS treatment. P < 0.01 by log-rank test; n = 6 each group. (B) Gross morphology and colonic macroscopic damage score of the large intestines on day 5. (C) H&E staining of colon sections and histological damage score on day 5. Original magnification 100x. *P < 0.05; **P < 0.01 vs. PBS; n = 6 in each group. (D) Western blot analyses of mucosal PUMA protein and caspase 3 activation on day 3. (E) Blood pressure changes following aliskiren treatment. **P < 0.01 vs. corresponding PBS. All gels were run under the same experimental condition and gel images are cropped for concise presentation.