Figure 5
From: Activation of the Renin-Angiotensin System Promotes Colitis Development
Chronic Ang II infusion promotes colitis.
WT mice were infused with PBS or Ang II by osmotic minipumps for 15 days. TNBS was given on day 7 after infusion. (A) Body weight changes (% of original body weight) over time (days) following TNBS treatment. P < 0.01 by log-rank test; n = 6. (B) Gross morphology and macroscopic damage score of the large intestine on day 6. (C) H&E staining of colon sections and histological score on day 6. Original magnification 100x. ***P < 0.001 vs. PBS; n = 6 in each group. (D) Mucosal pro-inflammatory cytokine production quantified by qPCR on days 3 after TNBS treatment. **P < 0.01; ***P < 0.001 vs. PBS. (E) Western blot analyses of mucosal PUMA and p53 proteins and caspase 3 activation on day 3. All gels were run under the same experimental condition and gel images are cropped for concise presentation.
