Figure 6
From: Reciprocal voltage sensor-to-pore coupling leads to potassium channel C-type inactivation
F416C in the pore domain affects molecular motions within the VSD.
(a) ON gating currents at 0 mV. Holding voltage VH = −80 mV. (b) Gating currents of F416C/W434F. VH = −80 mV. (c) Charge vs voltage of F416C/W434F calculated from ON gating currents. VH = 0 mV (black symbols), VH = −80 (white symbols). The continuous curves are best fits to Eq. 2 with a shared slope value. V½(−80 mV) = −61.0 mV, V½(0 mV) = −77.5 mV. The dashed curve is the best fit of a sum of two Boltzmanns’ curves (Eq. 2), where V½ for one component was fixed to −77.5 mV. V½ for the other component was determined to −58.3 mV. (d) OFF gating currents at −80 mV after 60 ms at 0 mV. (e) Gating currents of F416C/W434F. VH = 0 mV. (f) Charge vs voltage of W434F calculated from ON gating currents. VH = 0 mV (black symbols), VH = −80 mV (white symbols). The continuous curves are best fits to Eq. 2 with a shared slope value. V½(−80 mV) = −36.6 mV; V½(0 mV) = −64.9. All the recordings were done at pH 7.4.
