Figure 3

Effect of LPS/PepG co-administration and treatment with FTY720 on cardiac function and S1P serum levels in C57BL/6J wild type mice.

(a) Representative M-mode echocardiograms and legend of all groups studied. (b) Percentage ejection fraction, (c) fractional shortening (d), fractional area change were assessed via echocardiography and (e) S1P serum levels were assessed by LC/MS/MS 18 h subsequent to vehicle administration (sham) or LPS/PepG co-administration in 2-month-old male C57BL/6J wild type mice. At 1 h after LPS/PepG challenge mice were treated either with FTY720 (0.1 mg/kg) or vehicle (10% DMSO). The following groups were studied: C57BL/6J sham + vehicle (n = 8); C57BL/6J sham + FTY720 (n = 3); C57BL/6J LPS/PepG + vehicle (n = 14); C57BL/6J LPS/PepG + FTY720 (n = 15). Data are expressed as means ± SEM for n number of observations. *P < 0.05 vs. C57BL/6J LPS/PepG + vehicle (Kruskall-Wallis test with Dunn´s multiple comparisons test).