Figure 8
Effect of cecal ligation and puncture (CLP) and treatment with FTY720 on Akt, eNOS and ERK1/2 phosphorylation in murine heart tissue.
At 1 h after CLP or sham operation, mice were treated with either FTY720 (0.1 mg/kg) or vehicle. Signaling events in heart tissue were assessed at 24 h. Each immunoblot is from a single experiment and is representative of three separate experiments. All values were corrected for the corresponding β-actin band. Densitometric analysis of the bands is expressed as relative optical density (OD) of (a) phosphorylated Akt (pSer473) corrected for the corresponding total Akt content (Σ Akt) and normalized using the related sham band; (b) phosphorylated eNOS (pSer1177) corrected for the corresponding total eNOS content (Σ eNOS) and normalized using the related sham band and; (c) phosphorylated ERK1 (pThr202/pTyr204) and ERK2 (pThr185/pTyr187) corrected for the corresponding total ERK1 or ERK2 content (ΣERK1 or Σ ERK2) and normalized using the related sham band. Data are expressed as mean ± SEM for n number of observations. *P < 0.05 vs. CLP + vehicle (Kruskall-Wallis test with Dunn´s multiple comparisons test).
