Figure 3
The anti-fibrotic role of SAB in cultured MRC-5 fibroblasts stimulated with TGF-β.
(A) Growth curves (measured as Cell Indices over time) of MRC-5 fibroblasts treated with vehicle or with TGF-β, SAB, or both, were measured with the xCELLigence system. (B) Relative transcript levels of COL1A1, COL1A2, COL3A1, CTGF, PAI-1, TGF-β and α-SMA in the different treatment groups. (C) The protein level of α-SMA in the different treatment groups was determined by immunoblot and analyzed by densitometry. (D) The total soluble collagen in cell culture supernatants was quantified using the Sircol collagen assay kit. Data is presented as mean ± SD of three samples and compared by Student’s t test or one-way ANOVA test; *P < 0.05, **P < 0.01.
