Figure 4
The effects of SAB on the Smad signaling pathway and on the MAPK signaling pathway.
(A) The effect of SAB on TGF-β-induced SBE4 reporter gene activity was measured using a dual-luciferase reporter assay system. (B–E) MRC-5 fibroblasts were pretreated with SAB (50 μg/ml) for 24 hours and then treated with TGF-β (10 ng/ml) for another 30 min. Phosphorylation of Smad3 (B), ERK (C), JNK (D) and p38 (E) was determined by western blot and analyzed by densitometry. Data are presented as mean ± SD of three samples and compared by Student’s t test or one-way ANOVA test; *P < 0.05, **P < 0.01.
