Figure 2 | Scientific Reports

Figure 2

From: Interleukin-1β induced Stress Granules Sequester COX-2 mRNA and Regulates its Stability and Translation in Human OA Chondrocytes

Figure 2

Localization of marker proteins in IL-1β induced stress granules in OA chondrocytes.

Human OA chondrocytes were seeded in 8-well chamber slides (0.05 × 106 OA chondrocytes/well) for 2 to 3 days and treated with either IL-1β or H2O2 for 1 hour. The OA chondrocytes were fixed, permeabilzed and immunostained with primary antibodies specific TIA-1 (mouse) and G3BP1 (rabbit) (a) or TIAR (goat) G3BP1 (rabbit) (b) followed by anti-mouse/goat AlexaFluor 546 and anti-rabbit AlexaFluor 594 secondary antibodies. The nuclei were visualized by anti-fade mounting medium containing DAPI and images were acquired using an Olympus FV1000 confocal microscope. Treatment with H2O2 was used as positive control for SGs assembly.

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