Figure 5

PPARγ colocalizes with neurons and astrocytes in the adult mouse brain.

Note the clear absence of colocalization between PPARγ and microglia. Representative images of cell type specific stains color-coded in red (left panels), PPARγ color-coded in green (center panels) and merged images (right panels). (a–c) PPARγ colocalizes with NeuN in the nucleus and cytoplasm. (d–f) PPARγ colocalizes with GFAP only in the astrocytic processes. (g–i) PPARγ does not colocalize with Iba1. Arrows indicate positive colocalization examples and boxes represent negative colocalization. Confocal (63×), scale bar = 10 μm.