Figure 2: Over-expression of miR-320a induces resistance to 5-FU in pancreatic cancer cells.

(A) RT-PCR results showed the expression of miR-320a in PANC-1 cells after lentivirus infection with lenti_GFP and lenti_miR-320a. (B) Representative curves of growth-inhibitory effects of 5-FU in PANC-1 cells which infected with lenti_miR-320a and lenti_GFP. (C) Representative curves of growth-inhibitory effects of gemcitabine in PANC-1 cells which infected with lenti_miR-320a and lenti_GFP. (D) RT-PCR results of the expression of miR-320a in PATU8988 cells after lentivirus infection with lenti_GFP and lenti_miR-320a. (E) Representative curves of growth-inhibitory effects of 5-FU in PATU8988 cells after infecting with lenti_miR-320a and lenti_GFP. (F) Representative curves of growth-inhibitory effects of gemcitabine in PATU8988cells which infected with lenti_miR-320a and lenti_GFP. (G,H) Real-time PCR assay was conducted to detect the expression of “EMT” markers in PANC-1 cells and PATU8988 cells. Left panel: the expression of β-cadherin, E-cadherin, Right panel: the expression of Fibronectin N-cadherin, Vimentin, ZEB1, ZEB2 and Snail in PANC-1 cells. All data are presented as the means ± SD. Student’s t test (two-tailed) was performed to analyze data from the experiments in triplicate. P values < 0.05 were considered significant, as indicated by the asterisks (*p < 0.05, **p < 0.01, ***p < 0.001).