Figure 5: PDCD4 is a direct target of miR-320a in pancreatic cancer cells.

(A)The prediction of the binding between miR-320a and PDCD4 by Pictar. (B) Relative luciferase activity of the indicated PDCD4 reporter constructs in 293T cells. Error bars presented standard deviation obtained from three independent experiments. (C) RT-PCR showed the expression of miR-320a in PANC-1 cells after lentivirus infection with lenti_GFP and lenti_miR-320a. (D) Western blot analysis showed PDCD4 protein levels in PANC-1 after miR-320a over expression in pancreatic cancer cells. (E) RT-PCR results about the expression of miR-320a in PATU8988 cells after lentivirus infection with lenti_GFP and lenti_miR-320a. (F) Western bot analysis showed PDCD4 protein levels in PATU8988 after miR-320a over expression in pancreatic cancer cells. All data are presented as the means ± SD. Student’s t test (two-tailed) was performed to analyze data from the experiments in triplicate. P values < 0.05 were considered significant, as indicated by the asterisks (*p < 0.05, **p < 0.01, ***p < 0.001).