Figure 5
From: The transcriptional regulator GalR self-assembles to form highly regular tubular structures
CD spectra of 3.8 μM GalRWT (A) and GalRT322R (B) diluted in buffers containing the indicated [NaCl] and incubated for 1 hour before the spectra were recorded. Arrows indicate increased [NaCl] from top to bottom, and the double-headed arrow in (A) indicates the separation of spectra below (top) and above (bottom) 400 mM [NaCl]. (C) Amount (% of total) of the three different CCA-obtained pure spectra plotted against [NaCl] giving rise to a three-stage model. (D) Number based size distribution of GalRWT obtained using DLS at different [NaCl]. GalRT322R was used with 250 mM NaCl. 10 mM D-galactose was used with 500 mM NaCl.
