Table 3 Primer sequences used for RT-qPCR validations.
From: Comparative transcriptomic analysis of human and Drosophila extracellular vesicles
Homo sapiens sequences | ||
Forward primer | Reverse primer | |
TPT1 | 5′-CACGATGAGATGTTCTCCG-3′ | 5′-TCCTACTGACCATCTTCCC-3′ |
PABPC1 | 5′-CACTGGCATGTTGTTGGAG-3′ | 5′-CTTCATCAACCTTAGAACGGAG-3′ |
ATF4 | 5′-ATGATTACCTGGAGGTGGC-3′ | 5′-CTCCTTGCTGTTGTTGGAG-3′ |
PTBP1 | 5′-AGTTCTTCCAGAAGGACCG-3′ | 5′-GTTGTGCAGGTCAATGAGG-3′ |
HDGF | 5′-GTGACGGTGATAAGAAGGG-3′ | 5′-TTTAGGAGAGTCCTCCAGC-3′ |
G3BP1 | 5′-GTAGAGGAACCTGAAGAAAGAC-3′ | 5′- ATGTCATTACTGACAACTGCC-3′ |
BRAF | 5′- CTATTGGACAAATTTGGTGGG-3′ | 5′-GTATTCTTCATAGGCCTCCAG-3′ |
Drosophila melanogaster sequences | ||
Forward primer | Reverse primer | |
arc1 | 5′-TAGAAGGTATCAGCGACGAG-3′ | 5′-GCCATACCGTAGAACAGCA-3′ |
pabp2 | 5′-GCATACATTGAGTTTGGTTCC-3′ | 5′-CGACATTACCTTTATTTGACGC-3′ |
sqd | 5′-CACGGCAAGATCTTTGTCG-3′ | 5′-TCCACCTCGACGATATTGC-3′ |
cam | 5′-GAAACTCACAGACGAGGAG-3′ | 5′-CATCATAGTCACGAATTCTTCG-3′ |
rin | 5′-CAAGGGTGACTTTGAGCAG-3′ | 5′-GACATTTCCGAAGCGTGAG-3′ |
hmu | 5′-TCTACTGGACGGACTCCTC-3′ | 5′-TATTTGAAGAGACGGCCGG-3′ |
imp | 5′-CTCTACGAATAAGGGTGAACTC-3′ | 5′-CGTCCAATCAAATTGTTGTGG-3′ |
