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Figure 1

From: Ultrastructural analysis of the decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty in a rabbit model

Figure 1

Histological and scanning electron microscopy (SEM) micrographs of the decellularized corneas.

H&E and Masson’s trichrome staining of native cornea (a,e) and corneas treated with SDS (b,f), Triton X-100 (c,g) and high-hydrostatic pressure (HHP) (d,h). Histological analysis showing stratified epithelium and keratocytes in native cornea and no cell nuclei or debris in the decellularized cornea prepared by HHP. SEM observation of stroma in the native cornea (i) and corneas treated with SDS (j), Triton X-100 (k) and HHP (l) showing the similarity of lamella structure between native and decellularized cornea prepared by HHP. Scale bars: 100 μm in (a–h); 30 μm in (i–l).

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