Figure 3

Circular dichroism (CD) spectroscopy and differential scanning calorimetry (DSC).
CD spectra for collagen under various conditions (a) and Rpn values of collagen molecules estimated from CD spectra (b). Differential scanning calorimetry (DSC) analyses of the corneas treated with the high hydrostatic pressure (HHP) procedure (c). The Rpn value is expressed as the ratio of the positive peak intensity to the negative peak intensity.