Figure 2 | Scientific Reports

Figure 2

From: LTP-1, a novel antimitotic agent and Stat3 inhibitor, inhibits human pancreatic carcinomas in vitro and in vivo

Figure 2

Effect of LTP-1 on tubulin destabilization and G2/M regulatory proteins.

(A) Effect of LTP-1 on microtubule assembly in vitro. Purified tubulin was suspended in reaction buffer incubating at 37 °C with GTP in the absence or presence of LTP-1, paclitaxel or vincristine. Assembly of microtubules was determined by measuring absorbance at 340 nm using a spectrophotometer every 1 min for 30 min. (B) Effect of LTP-1 on tubulin distribution. AsPC-1 cells were treated with DMSO, LTP-1, paclitaxel or vincristine for 24 h. After treatment, microtubules were stained with β-tubulin and FITC-conjugated anti-mouse IgG (green fluorescence). Nuclear DNA was visualized by DAPI staining (blue fluorescence). Microtubule networks were analyzed with the Leica TCS SP2 Spectral Confocal System. Scale bar, 10 μm. (C) Effect of LTP-1 on G2/M cell cycle regulatory proteins. (D) Time-dependent effect of LTP-1 on Histone 3 phosphorylation. Both cells were treated with LTP-1 or vincristine for (C) 24 h or (D) indicated time interval and then cell lysate were subjected to western blot analysis with indicated antibodies.

Back to article page