Figure 3: BI-1 regulates fatty acid synthesis gene expression and ApoB lipoprotein secretion.
From: Bax Inhibitor-1 regulates hepatic lipid accumulation via ApoB secretion
(a) BI-1 WT and BI-1 KO mice (8 weeks old, n = 5) were fed with HFD for 1- and 8-weeks. After mRNA isolation, the transcription of genes involved in triglyceride and cholesterol biosynthesis was measured in the livers of HFD-fed mice. The mRNA levels of genes encoding FAS, SCD1, ACC1, HMGCS, and HMGCR in the livers of the mice were determined by real-time RT-PCR. (b) Immunoblot analysis of plasma ApoB from 1- and 8-weeks HFD fed BI-1 WT or KO mice. The relative levels of plasma ApoB were measured compared to the mean levels in mice fed a normal diet. (Mean ± SEM; n = 5, *p < 0.05, #p < 0.001). HFD; high-fat diet, FAS; fatty acid synthase, SCD1; stearoyl CoA desaturase 1, ACC1; acetyl-CoA carboxylase, HMGCS; 3′-hydroxylmethyl glutaryl coenzyme A synthetase, HMGCR; 3′-hydroxylmethyl glutaryl coenzyme A reductase.
