Figure 2
From: Leptospiral outer membrane protein LipL32 induces inflammation and kidney injury in zebrafish larvae
Leptospira Shermani induced acute tubular injury in zebrafish larvae.
Transverse histological sections (H&E stain) from control (A,D,G), L. Patoc-treated (B,E,H) and L. Shermani-treated larvae (C,F,I). Note the marked swelling of the proximal pronephric ducts (circles) in L. Shermani-treated larvae compared with L. Patoc-treated larvae and controls. The differences were not evident in the distal pronephric ducts. Living larvae were incubated in E3 media containing L. Shermani or L. Patoc (1 × 106 CFU/ml) from 24 hpf to 48 hpf. Control larvae were incubated with E3 buffer only. Representative micrographs from the level of the glomerulus (A–C), proximal pronephric duct (D–F) and distal pronephric duct (G–I) are shown. Circles indicate the location of the pronephric duct. Pt, pronephric tubule; Glm, glomerulus; Scale bar, 10 μm. (J,K) Quantification of the area of pronephric ducts. *P < 0.05 compared to control. n = 6 from three larvae in each group. (L) Diagram of transverse sections illustrating the structure of zebrafish pronephros at 48 hpf.
