Figure 1

Relative expression of three trehalase (TRE) genes, activity of TRE1 and TRE2 and content of trehalose after double stranded RNA (dsRNA) injection of Nilaparvata lugens fifth instar larvae.
(A–C) Changes in TRE1-1, TRE1-2 and TRE2 expression at 48 h and 72 h after dsTRE1-1, dsTRE1-1, dsTRE2, dsTREs and dsGFP injection. (D,E) Changes in TRE1 and TRE2 activity at 48 h and 72 h after dsTRE1-1, dsTRE1-1, dsTRE2, dsTREs and dsGFP injection. (F) Changes in trehalose content at 48 h and 72 h after dsTRE1-1, dsTRE1-1, dsTRE2, dsTREs and dsGFP injection. Bars represent means. Error bars indicate one standard error of the mean. ‘*’ Indicates significant differences at p < 0.05 and ‘**’ indicates significant differences at p < 0.01. Green fluorescence protein (GFP) was used as control.