Table 2 Primers used for double stranded RNA synthesis.

From: Functional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using RNA interference

Primer Name

Forwad Primer (5′-3′)

Reverse Primer (5′-3′)

Length (bp)

NLTRE1-1

ATGTCCCAATGTGCCATTCACAG

TCACGTACCATTCAAGAATATGTC

1803

DSNLTRE1-1

GATGCAATCAAGGAGGTGTTATGGC

CGTATTCACCTCCACCTCCGT

451

DSNLTRE1-1-T7

T7-GATGCAATCAAGGAGGTGTTATGGC

T7-CGTATTCACCTCCACCTCCGT

 

NLTRE1-2

ATGAAGGCAAAAAAACATGGTGAGGCC

CTATAAATGATGCATGAAACGTTTTTCC

1506

DSNLTRE1-2

AGATGAAGGCATGTGGTTCG

CATCGATTCGCCAACTGGTAAGC

321

DSNLTRE1-2-T7

T7-AGATGAAGGCATGTGGTTCG

T7- CATCGATTCGCCAACTGGTAAGC

 

NLTRE2

ATGACGACTGTTAATCTCTTCACGGT

CTAGTCACATGGTTTTAGATCCTTC

1998

DSNLTRE2

CCAACTGCTATGACACCGACAAG

GGGTTCAGATCCTGCCGTCGCT

440

DSNLTRE2-T7

T7-CCAACTGCTATGACACCGACAAG

T7-GGGTTCAGATCCTGCCGTCGCT

 

DSNLGFP

AAGGGCGAGGAGCTGTTCACCG

CAGCAGGACCATGTGATCGCGC

688

DSNLGFP-T7

T7-AAGGGCGAGGAGCTGTTCACCG

T7-CAGCAGGACCATGTGATCGCGC

 
  1. T7 sequence: GGATCCTAATACGACTCACTATAGG.
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