Table 3 Primers used for quantitative real-time PCR (qRT-PCR) of genes involved in the chitin metabolism pathway.
Gene | Genebank number | Forwad (5′-3′) | Reverse (5′-3′) |
|---|---|---|---|
NLTRE1-1 | FJ790319 | GCCATTGTGGACAGGGTG | CGGTATGAACGAATAGAGCC |
NLTRE1-2 | KU556829 | GATCGCACGGATGTTTA | AATGGCGTTCAAGTCAA |
NLTRE2 | GQ397451 | TCACGGTTGTCCAAGTCT | TGTTTCGTTTCGGCTGT |
NLHK | KU556830 | GGTGCGAGAAGAAGTGAAG | GTGAAACCCATTGGTAGAGT |
NLGPI1 | KU556831 | CCTGCCACCAGTCATAACCC | CTTGTGAGGAAGGATGCGTTT |
NLGPI2 | KU556832 | ATCAGCCGTAGCCAAGCAC | AAGCCGATAGCAGACCACAAC |
NLGFAT | KU556833 | CCTCCCAGTTCATCTCGC | CCAAGTTCTTCAAACCCTTTAT |
NLGNPNA | KU556834 | TGAGCTGCTGAAGACACT | CCTGAATAACGGTGATGTA |
NLPGM1 | JF330414 | AACGAGACGGTGGGAGAC | TCCTGGTAAGTGTTGAGCC |
NLPGM2 | KU556835 | AGAGGAAGGTTGGGAGTG | CATAATTCGCGGAGATAAG |
NLUAP | JF330415 | ACGACAGATTAAAGCCGATAC | TACCTTGTCCACCAGCCA |
NLCHS1 | AEL88648 | CCGCAAACGATTCCTACAGA | AGGTCCTTGACGCTCATTCC |
NLCHS1a | JQ040014 | TGTTCTTGCTACAACTCAATAAA | ACACCAATCCGATAGGCTC |
NLCHS1b | JQ040013 | GCTGTCTTTGCTTTCTTCAT | ACACCAATCCGATAGGCTC |
NLCht1 | AJO25036 | AGGTGGTTAGGGACGAGGAG | TGCGCTTGACATAGTTGGACT |
NLCht2 | AJO25037 | GCAGATTTCTGGACAGGGAA | TGACGCACAAGCGGGAAG |
NLCht3 | AJO25038 | CTACACCTCTGGCTAAACTCGG | AACTTGTCCTTGCGGCTGAT |
NLCht4 | AJO25039 | TTGAGGAGGTTCACGGGTCT | CCTTACTGGAAACGAGGTTGG |
NLCht5 | AJO25040 | AAAGCGTTCGTGATGAAATAGC | GATCCTTTGCCTCAATCCAAT |
NLCht6 | AJO25041 | GCTGGTAAGGAGATGCTATTCG | GTGGTTCTAAGGCTGGCTGTC |
NLCht7 | AJO25042 | CTACTCTGCCATCCCATTCCT | GTCTGGGTTTCTTCACTTCCTG |
NLCht8 | AJO25043 | GAACAAAGTGCAAACTCAGTCC | CACCTTCTGTGGCTTCTGG |
NLCht9 | AJO25044 | GTGCGGTATTGGTTGAAGAGG | GGTATAACGTGATTCCGAGCC |
NLCht10 | AJO25045 | CAAGCCAATACCCAACAAAC | ACAGCAAATCCATAGAGCACA |
NLIDGF | AJO25056 | AAAAGAACGAGGAGGAGGG | TTGCTTGAGGATGGGGTAC |
NLENGase | AJO25057 | TGTGGCAAGACTTCGTTA | ATGGGAGGGTTGGGATAG |
NL-18S | – | CGCTACTACCGATTGAA | GGAAACCTTGTTACGACTT |
