Figure 2

Effect of 58-F on cell viability and proliferation.

(A) The effects of different concentrations (0–2500 μM) of H₂O₂ on cell viability were determined. Four thousand cells seeded in 96-well plates were treated with H₂O₂ for 2 h. (B) The effects of different concentrations (1–200 μM) of 58-F on cell viability were determined. Four thousand cells seeded in 96-well plates were treated with 58-F for 24 h (C) The effects of 58-F on changes in cell viability induced by H₂O₂ at different time intervals were measured. Four thousand cells in 96-well plates were treated with 50 μM 58-F and 500 μM of H₂O₂ was added 2 h before the end of incubation. (D) The effect of different concentrations (0.1–100 μM) of 58-F on changes in cell viability induced by H₂O₂ were measured. (E,F) For the BrdU incorporation assay, 4000 cells seeded in a 96-well plate were treated with 50 μM of 58-F for 24 h and with 500 μM H₂O₂ for an additional 8 h. A final concentration of 10 μM 5-bromo-2-deoxyuridine was supplemented in the medium for 24 h (scale bar = 50 μm). All data are presented as the mean ± SD. Statistical analysis was performed using Student’s t test. (*p < 0.05, **p < 0.01).