Figure 3

Effect of 58-F on cell death.
(A) For the Annexin V/PI assay with FACS, the cells were treated with 50 μM of 58-F for 12 h or 36 h followed by an additional 2 h with 500 μM H2O2. (B–F) Bax and caspase3 protein levels were examined by Western blotting and were quantified. All data are presented as the mean ± SD. Statistical analysis was performed using Student’s t test. (*p < 0.05, **p < 0.01).