Figure 7
lysosomal acidification inhibition leads to cell death.
(A) AO staining (scale bar = 25 μm) results are shown, the cells were treated with 100 nM BafA1 for 24 h or 100 μM NH4Cl for 2 h. All data are presented as the mean ± SD. Statistical analysis was performed using Student’s t test. (*p < 0.05, **p < 0.01). (B) Quantification of the AO staining is shown. (C) Cell viability: The cells seeded in 96-well plates were incubated with 100 nM of BafA1(V-ATPase inhibitor) for 24 h followed by H2O2 for 0.5 h, 2 h, 8 h and 24 h. (D) Cell viability: Cells seeded in 96-well plates were incubated with 100 μM of NH4Cl for 40 min. All data are presented as the mean ± SD. Statistical analysis was performed using Student’s t test. (**p < 0.01). (E) For the Annexin V/PI assay with FACS, the cells were treated with 100 μM NH4Cl for 2 h.
