Figure 5: (a)
From: TRPV4 Regulates Breast Cancer Cell Extravasation, Stiffness and Actin Cortex
Effect of Trpv4 knock down on metastasis of 4T1 cells. Top panel: H&E staining of lung tissue sections from mice injected with 4T1 cells transfected with control or Trpv4-specific siRNA. ‘T’ indicates tumor nodules and ‘N’ indicates normal lung tissues. Three magnifications (40x, 200x and 600x) of the representative H&E images (n = 10 for each groups). Bottom panel: Table showing the number of mice in the control or Trpv4knocked-down condition that possessed the categorical numbers of nodules. (b) Upper panel: Representative IHC images showing the expression of TRPV4 in the nodules of lungs of mice from control and Trpv4-knocked down conditions. Lower Panel: Box plots showing the expression of TRPV4 in the metastatic nodules in the lungs from mice in the control and Trpv4-knocked down conditions. (c) Top panel: The size of all the nodules in lungs of every mouse in control and Trpv4-knocked down conditions were measured and the numbers of nodules within 3 size groups were plotted. Bottom left panel: Box plots showing the distribution of nodules size in the mice within the control and Trpv4-silenced conditions. ‘n’ refers to the sample size of the nodules in each condition. Bottom right panel: Box plots of the total number of nodules in each mouse within the control and Trpv4-silenced condition. ‘n’ refers to sample size of mice.
