Figure 6: Micropipette aspiration experiment to measure cancer cell rigidity following Trpv4 knock down or overexpression in 4T07 and MB468 cells, respectively.
From: TRPV4 Regulates Breast Cancer Cell Extravasation, Stiffness and Actin Cortex
(a) The percentage of 4T07 cells that formed blebs before and after Trpv4 silencing. (b) Arrows show that bleb formed in control cells but not cells transfected with S1- and S3- Trpv4-specific siRNAs. The minimum pressure at which bleb developed in (c) 4T07 cells and (d) MB468 cells were plotted. (e) The shear modulus (Pa) in the MB468 cells was determined from using the linear elastic solid model. P values from unpaired Student’s t-test are shown.
