Figure 7: TRPV4 overexpression affected the cytoskeleton network compared to the control.

(a) Lysates from control or stable TRPV4 MB468 transductants untreated or treated with deionized water for 3 min were immunoblotted with the indicated antibodies. Data shown are mean ± s.d. (n = 3) (b) Insoluble (contains F-actin) and soluble fractions (contains G-actin) isolated from the total lysates were probed for Actin. Data shown are mean ± s.d. (n = 4). (c) Lysates from control or stable TRPV4 MB468 transductants were immunoblotted with the phospho-Cofilin or total Cofilin antibodies. (d) Lysates from MB468-Vector and MB468-TRPV4 cells not treated or treated with deionized water were probed for total VASP and phospho-VASP antibodies. Protein expression levels were normalized and quantified as shown by the bar chart. GAPDH were used as a loading control. Average values from three biological repeats (n = 3, ±s.d.) and p values from unpaired Student’s t-test are shown. (e) MB468-Vector and MB468-TRPV4 cells were not pre-treated or treated with RR for an hour before the replacement of the media with deionized water or fresh growth medium. Densitometry was performed. Protein expression levels were quantified and presented as relative change in E-cadherin expression levels in the various conditions compared to their respective untreated samples. β-Tubulin were used as a loading control. Average values from three biological repeats (n = 5, ±s.d.) and p values from unpaired Student’s t-test are shown.