Figure 3 | Scientific Reports

Figure 3

From: Angiotensin-converting enzyme 2 prevents lipopolysaccharide-induced rat acute lung injury via suppressing the ERK1/2 and NF-κB signaling pathways

Figure 3

Effects of ACE2 and different treatments on LPS-induced secretion of inflammatory mediators in BALF.

(A) LPS administration caused a significant increase of Ang II, which was markedly attenuated by ACE2 overexpression and aggregated by ACE2 knockdown. (B) LPS exposure also caused a significant increase of Ang-(1-7), which was further up-regulated by ACE2 overexpression and reduced by ACE2 knockdown. Pretreatment with A779 or MLN-4760 both reversed the changes of Ang II and Ang-(1-7) that resulted from ACE2 overexpression. (C–F) The levels of TNF-α, IL-1β, IL-6 and IL-10 were significantly increased after 8 hours of LPS injection. ACE2 overexpression markedly suppressed the TNF-α, IL-1β and IL-6 secretion and increased IL-10 level, which were noticeably abolished by pretreatment with A779 or MLN-4760. The increased levels of TNF-α, IL-1β IL-6 and IL-10 caused by LPS administration were all further promoted by ACE2 knockdown. Data are represented as mean ± SD. *p < 0.05, versus control group; #p < 0.05, versus LPS group; $p < 0.05, versus ACE2 group (n = 6, per group).

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