Figure 1: Effects of different human serum on leptin production in 3T3-L1 adipocytes. | Scientific Reports

Figure 1: Effects of different human serum on leptin production in 3T3-L1 adipocytes.

From: Association of Increased Serum Leptin with Ameliorated Anemia and Malnutrition in Stage 5 Chronic Kidney Disease Patients after Parathyroidectomy

Figure 1

The differentiated adipocytes were treated with 10% human serum media for 24 h. The serum-stimulated groups included healthy controls (CTL), No-PTX patients (No-PTX), preoperative (PTX or Pre PTX), and follow-up postoperative PTX patients (Post PTX) (n = 5). Leptin production was measured using ELISA or Western blot analysis. (A,D) Leptin levels in medium assessed by ELISA. (B,E) Leptin protein in cytoplasm detected by immunoblotting. (C,F) Densitometry analysis of immunoblotting images. The data from all the groups were normalized corresponding to the control at each time of exposure, respectively. Results were shown as mean ± SD, and error bars were pooled from at least three independent experiments.

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