Figure 2: Effects of high PTH, Ca or P on leptin production and Akt phosphorylation in 3T3-L1 adipocytes. | Scientific Reports

Figure 2: Effects of high PTH, Ca or P on leptin production and Akt phosphorylation in 3T3-L1 adipocytes.

From: Association of Increased Serum Leptin with Ameliorated Anemia and Malnutrition in Stage 5 Chronic Kidney Disease Patients after Parathyroidectomy

Figure 2

The differentiated adipocytes were treated with high levels of PTH (0.1 nM, 1.0 nM), high Ca (2.5 mM, 3.5 mM) or high P (2.5 mM, 3.5 mM) for 24 h. Leptin production was measured using ELISA or Western blot analysis. Akt phosphorylation and Akt expressions were detected using Western blot analysis. (A,D,E) Leptin levels in medium assessed using ELISA. (B,F) Leptin protein detected by immunoblotting. (C,G) Densitometry analysis of immunoblotting images. The data from all these groups were normalized corresponding to the control at each time of exposure. Results were shown as mean ± SD, and error bars were pooled from at least three independent experiments. CTL, controls; Ca, calcium; P, phosphorus; PTH, parathyroid hormone.

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