Table 1 Sequences of PCR primers.
No | Purpose | primer | 5′ to 3′ sequence |
|---|---|---|---|
1 | Partail cDNA PCR | ERα F1 | TATGTGCCCAGCAACCAATC |
ERα R1 | CCTTCGTTCCTGTCCAGTATG | ||
2 | 5′ RACE PCR | ERα R2 | CCTCCGATTTCTGTCTATGGTA |
ERα R3 | GCCCACTTCATAACACTTCCTA | ||
3 | 3′ RACE PCR | ERα F2 | TGAGATCTTCGACATGCTGC |
ERα F3 | TGGCTGTGTTGAGGGCATGG | ||
4 | Quantitative RT-PCR | ERα F4 | GCCAGGCTTGTCGTCTTAGG |
ERα R4 | TCCTTCACCACCGCCATTA | ||
5 | β-Actin | F1 | ACTTTGAGCAAGAAATGGGAACT |
R1 | GGACTCAGGGCAACGGAAC | ||
6 | ISH probe | ERα F5 | GACTATGCTGCTCCAACCC |
ERα R5 | CCCAAATTCCCCCTACTACA |
