Figure 1 | Scientific Reports

Figure 1

From: Intrinsic hepatocyte dedifferentiation is accompanied by upregulation of mesenchymal markers, protein sialylation and core alpha 1,6 linked fucosylation

Figure 1

Primary rat hepatocytes undergo an EMT as a function of time in culture.

10X phase contrast microscopy of PRH at 24 (A) or 96 hours (B) post culture. (C,D) are magnified images highlighting either typical hepatic architecture with di-nucleated cells and bile canaliculi at the early time point (C) or where this is lost at the later time point (D). (E) Protein level of E-cadherin (E-Cad), N-cadherin (N-Cad), caveolin-1 (Cav), vimentin (Vim) and alpha smooth muscle actin (α-SMA) a function of time in culture. Proteins were detected by immunoblot analysis at indicated times post-culture; lane M is the marker lane. Actin is shown as a loading control. For Fig. 1E, Hep G2 lysates were used as a control as the vimentin and SMA antibodies cross react with human proteins. Figure 1E contains cropped images highlighting the change in E-cadherin (E-Cad), N-cadherin (N-Cad), caveolin-1 (Cav), vimentin (Vim) and alpha smooth muscle actin (α-SMA) as a function of time. Analysis of E-Cad, Cav and Vim were performed on the same membrane and thus under the same experimental conditions.

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