Figure 5 | Scientific Reports

Figure 5

From: Intrinsic hepatocyte dedifferentiation is accompanied by upregulation of mesenchymal markers, protein sialylation and core alpha 1,6 linked fucosylation

Figure 5

Increased fucosylation is observed with de-differentiated HCC by lectin staining with the N224Q AAL lectin.

Lectin histochemistry to detect fucosylated proteins was performed using the N224Q lectin on either normal (A,D) or HCC tissue (B,E) and (C,F). (A) 4X microscopy of four tissue spots of tumor-adjacent normal tissue. (B) 4X microscopy of four tissue spots of Grade I-II or II HCC tissue. Grade I is defined as well-differentiated (low grade) and Grade II is defined as moderately differentiated (intermediate grade). (C) 4X microscopy of four tissue spots of Grade II-III or Grade III HCC tissue. Grade III is defined as poorly differentiated tissue. (D) Microscopy of normal, tumor-adjacent tissue. (E) grade I-II HCC tissue, (F) grade III HCC tissue showing both a 4X image and a 10X image. In all cases lectin staining was performed using N224Q rAAL.

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